Each year, health care professionals around the world provide resuscitative care to over 13 million newborn infants at birth. While simulation-based education is recommended for health care professionals to train and assess their neonatal resuscitation competence, this approach is resource-draining and overall inaccessible. Therefore, most health care professionals are unable to maintain their clinical skills or knowledge. To address this barrier, we developed RETAIN, a simulation-based board game. RETAIN uses experiential game-based learning to teach neonatal resuscitation within an engaging educational environment. This case study describes the research plan we developed to evaluate the educational outcomes of playing the RETAIN board game. Our objectives were to evaluate the RETAIN board game for purposes of (a) training and (b) assessing neonatal resuscitation competence in a population of experienced neonatal health care professionals from a tertiary perinatal center. We measured short-term knowledge retention of the neonatal resuscitation algorithm using a pre-post-test observational study design. Furthermore, we examined the utility of RETAIN as a summative assessment tool of neonatal resuscitation competence using an observational study design. Finally, we quantitatively and qualitatively measured health care professionals’ opinions of RETAIN. This case study also discusses our experience conducting and troubleshooting clinical research in continuing health care education, including the importance of interdisciplinary decision-making, institutional partnerships, and conscientious recruitment of health care professional participants.